Medical Laboratory Journal

en Comparison of serum levels of zinc, copper, and selenium between patients with coronary artery disease and healthy controls; Selenium could be a new diagnostic biomarker بیوشیمی Biochemistry تحقيقي Original Paper Background: Although several clinical and laboratory diagnostic approaches have been developed for Coronary Artery Disease (CAD), rates of mortality and morbidity have remained high, which may be due to defective diagnostic markers. Here, we evaluated the serum levels of selected trace elements (Zn, Se, and Cu) in CAD patients and healthy subjects and assessed their diagnostic values in CAD. Methods: 53 cardiologist-approved CAD patients and 48 age and sex-matched healthy controls were recruited. The serum levels of Zn, Se, and Cu, were assessed using the atomic absorption method. GraphPad v.8.4 and SPSS v.18 software were used for statistical analyses. Results: The serum levels of Zn and Se were significantly lower in the CAD patients than in controls (P = 0.0008 and P < 0.0001, respectively). In contrast, CAD patients showed significantly higher levels of Cu (P = 0.0064). Concerning the ROC curve analyses, the area under the curve (AUC) for Zn was 0.6563 (P = 0.0069). Setting the cut-off value at 1.255 μg/mL gave a sensitivity of 56.60%, specificity of 66.67%, and likelihood ratio (LR) of 1.698. AUC for Se was 0.8595 (P<0.0001). The optimum cut-off value at 86.50 ng/ml level gave a sensitivity of 83.02%, specificity of 75.00%, and LR of 3.321. The AUC for Cu was 0.6557 (P = 0.0071). The optimum cut-off value at 1.225 μg/mL level gave a sensitivity of 62.26%, specificity of 64.58%, and LR of 1.758. Conclusion: Our study showed that selenium can be a biomarker with reliable diagnostic value for CAD. Trace elements, coronary artery disease (CAD), Lipid Profile, Diagnostic marker 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1644-1&slc_lang=en&sid=1 Mahrooyeh Haji-Abolhasan Varaminfard mahro.abolhasanifard8190@gmail.com 100319475328460032359 100319475328460032359 No Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran Mina Ebrahimi-Rad minebrad@pasteur.ac.ir 100319475328460032360 100319475328460032360 No Department of Biochemistry Seifoddin Javadian Javadian@yahoo.com 100319475328460032361 100319475328460032361 No Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran Mahmood Beheshti Monfared m_beheshti_m@yahoo.com 100319475328460032362 100319475328460032362 No Cardiovascular Department, Modares Hospital, Tehran, Iran Seyedeh Fatemeh Sadati Khalili fsadati8940@gmail.com 100319475328460032363 0009-0001-7259-4771 No Department of Biochemistry, Islamic Azad University of Central Tehran Branch, Tehran, Iran Reza Saghiri reza_saghiri@yahoo.com 100319475328460032364 100319475328460032364 Yes Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.