en بیوشیمی Biochemistry Research Article Research Article <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background and objectives:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Activation of adenosine A2a receptor has been shown to induce the growth and metastasis of cancer cells. The role of this receptor in esophageal cancer has not yet been determined. The present study aimed to investigate effects of an adenosine A2a receptor antagonist (3, 7-dimethyl-1-propargylxanthine) on growth of esophageal cancer cells. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Real-time polymerase chain reaction was performed to evaluate mRNA expression of the A2a adenosine receptor in KYSE-30 and YM-1 esophageal cancer cell lines. Effects of the antagonist on viability of the cells were evaluated by MTT assay.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> At low concentrations, the antagonist had no effect on cell viability. However, at concentrations &ge;200 &mu;M, the antagonist significantly reduced viability of both cell lines (<i>p</i><0.05). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> The results of this study indicate that the adenosine A2a receptor antagonist exerts inhibitory effects on KYSE30 and YM-1 cancer cells in a dose-dependent manner. Therefore, the use of this antagonist can be exploited as a therapeutic target for the treatment of esophageal cancer.</span></span></span></span></span></span></span> Cell Death,3,7-dimethyl-1-propargylxanthine,Esophageal Neoplasms, 37 42 http://mlj.goums.ac.ir/browse.php?a_code=A-10-840-1&slc_lang=en&sid=1 Parisa zeinali parisazeinali751@gmail.com 100319475328460023087 100319475328460023087 No Department of Biochemistry and Biophysics, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran marie saghaeian marie.saghaeian@yahoo.com 100319475328460023088 100319475328460023088 No Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Jahanbakhsh Asadi dr.asadi@goums.ac.ir 100319475328460023089 100319475328460023089 No Metabolic Disorders Research Center, Golestan University if Medical Sciences, Gorgan, Iran Seyyed Mehdi Jafari s.meh.jafari@goums.ac.ir 100319475328460023090 100319475328460023090 Yes Metabolic Disorders Research Center, Golestan University if Medical Sciences, Gorgan, Iran