en ميکروب شناسي Microbiology Research Article Research Article <strong>Background and objectives:</strong> Antibiotic resistance is a global health challenge that affects both individuals and the health system in many ways. The aim of this study was to evaluate the antibiotic resistance pattern in isolates from patients admitted to the intensive care unit (ICU) of a hospital in Qazvin, Iran.<br> <strong>Methods:</strong> This descriptive and retrospective study was performed on urine and blood samples collected from 1318 ICU patients in the Velayat Hospital of Qazvin (Iran) during 2017-2019. Data were collected from patients&rsquo; medical records. All statistical analyses were performed using SPSS software (version 25).<br> <strong>Results:</strong> Based on the findings, 65.2% of the samples were related to urinary tract infections and 34.7% to bloodstream infections. <em>Escherichia coli</em> (68.6%) and <em>Stenotrophomonas</em> (41.0%) were the most common bacteria isolated from urinary tract infections and bloodstream infections, respectively. Moreover, the rate of antibiotic resistance was higher among <em>Acinetobacter</em>, <em>Escherichia coli</em>, <em>Stenotrophomonas</em>, <em>Enterococcus</em> and <em>Pseudomonas </em>isolates.<br> <strong>Conclusion:</strong> The rate of drug resistance in isolates from ICU patients is alarmingly high and requires immediate attention. It is recommended to modify antibiotic prescriptions in the hospital based on the results of antibiotic resistance pattern, particularly for treatment of infections caused by <em>E. coli</em> and <em>Stenotrophomonas</em>. Drug resistance,Intensive care units,Hospitals, 31 37 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1026-1&slc_lang=en&sid=1 Fatemeh Bagherian fateme.bagherian@yahoo.com 100319475328460020972 100319475328460020972 No Department of Infectious Diseases, Clinical Research Development Unit, BouAli Sina Hospital, Qazvin University of Medical of Sciences, Qazvin, Iran Alireza Nikoonejad nikoonejad.anikoo@gmail.com 100319475328460020973 100319475328460020973 Yes Department of Infectious Diseases, Clinical Research Development Unit, Booalisina Hospital, Qazvin University of Medical of Sciences, Qazvin, Iran Abbas Allami allami9@yahoo.com 100319475328460020974 100319475328460020974 No Department of Infectious Diseases, Clinical Research Development Unit, Booalisina Hospital, Qazvin University of Medical of Sciences, Qazvin, Iran Samira Dodangeh sdodangeh@ymail.com 100319475328460020975 100319475328460020975 No Department of Medical Parasitology and Mycology, Children Growth Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran Layla Taha Yassen laylataha23@gmail.com 100319475328460020976 100319475328460020976 No Department of Medical Laboratory Techniques, Osoul Aldeen University College, Baghdad, Iraq Bahram Hosienbeigi Bahramhb2021@gmail.com 100319475328460020977 100319475328460020977 No Department of Medical Parasitology and Mycology, Qazvin, Iran