Medical Laboratory Journal

fa Investigating Association of Human-Specific Derived Alleles of CD33 and Other Genes with Lifespan of Iranians فیزیولوژی ورزشی Sport Physiology تحقيقي Original Paper <div>ABSTRACT              Background and Objectives: Aging is a multi-agent phenomenon due to prolonged inflammation and stress. CD33 or Siglec3 is a membrane receptor that acts against aging by inhibiting inflammatory reactions. The aim of this study was to evaluate a possible relationship between CD33 copy number and lifespan of an Iranian population.              Methods: The study included 50 individuals with cancer or Alzheimer's disease as the case group and 50 members of a family over 70 years old as the control group. Blood samples were collected and transferred to the laboratory. CD33 copy number was calculated using the QX100 Droplet Digital PCR system. A number of CD33 single-nucleotide polymorphisms including rs3865444, rs273634 and rs3852865 were genotyped using specific primers and the PCR method.              Results: The mean number of CD33 copies among the case group (7.78) was significantly lower (P<0.05) than control group (12.72). In the case group, the mean number of CD33 copies was 7.83 among men and 7.73 among women. In the control group, the mean number of CD33 copies was 12.73 among men and 12.71 among women.              Conclusion: CD33rSiglecs counteract random molecular damage, which is the main driver of aging. Therefore, the CD33rSiglec gene number may be correlated with longevity. Our results indicate that there may be a link between reduced CD33rSiglec copy number and development of diseases.              Keywords: Gene Copy Number, Siglec-3, CD33 Antigens, Cancer.</div> Gene Copy Number, Siglec-3, CD33 Antigens, Cancer. 19 25 http://mlj.goums.ac.ir/browse.php?a_code=A-10-353-3&slc_lang=fa&sid=1 Mehdi Kargar 100319475328460018816 100319475328460018816 Yes Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran Hoshang Jamali 100319475328460018817 100319475328460018817 No Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Mehdi Sadeghi 100319475328460018818 100319475328460018818 No Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran