Medical Laboratory Journal

en Distribution of Ambler Class A Β-lactamase Genes and Evaluation of Resistance Patterns in Multi-Drug and Extensively-Drug Resistant P. aeruginosa Clinical Isolates باکتری شناسی bacteriology تحقيقي Original Paper <div>ABSTRACT              Background and Objectives: Emergence and spread of multidrug-resistant (MDR) and extensively-drug resistant (XDR) Pseudomonas aeruginosa strains could complicate antipseudomonal chemotherapy. Dissemination of resistance genes, such as β-lactamases encoding genes by horizontal gene transfer can lead to development of multi-drug resistance in P. aeruginosa. The purpose of this study was to investigate the latest resistance patterns in MDR and XDR strains and evaluate Ambler class A β-lactamase gene distribution in P. aeruginosa clinical isolates.              Methods: One hundred molecularly and biochemically identified P. aeruginosa strains isolated from different clinical specimens were tested for sensitivity to 17 antibiotics using the Kirby-Bauer disk diffusion method. PCR was performed to detect bla TEM-1, bla SHV-1, bla REP-1 and bla VEB-1 genes. Results were analyzed using SPSS and NTSYSpc softwares.               Results: Based on the results of antibiogram, the highest rate of resistance was observed against amikacin (100%), aztreonam (83%), ceftazidime (55%), cefepime (55%) and netilmicin (48%). In addition, the frequency of MDR and XDR isolates was 95% and 5%, respectively. The blaSHV-1, bla TEM-1, bla PER-1 and bla VEB-1 genes were detected in 31%, 24%, 13% and 10% of the isolates, respectively.              Conclusion: Antibiotic resistance to β-lactam antibiotics and frequency of β-lactamase genes were relatively high in the study area. We also found that a significant proportion of XDR strains with different antibiotic resistance profile is isolated from tracheal specimens.              KEYWORDS: Pseudomonas aeruginosa, Beta-Lactamase, Multidrug Resistant, Extensively Drug Resistant.</div> Pseudomonas aeruginosa, Beta-Lactamase, Multidrug Resistant, Extensively Drug Resistant. 1 7 http://mlj.goums.ac.ir/browse.php?a_code=A-10-704-1&slc_lang=en&sid=1 Seyed Amin Enayatzadeh meymandi amin.enayatzadeh69@gmail.com 100319475328460018809 100319475328460018809 No Department of Biology, Faculty of Science, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran Laleh Babaeekhou babaeekhou@iiau.ac.ir 100319475328460018810 100319475328460018810 Yes Department of Biology, Faculty of Science, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran Maryam Ghane ghane@iiau.ac.ir 100319475328460018811 100319475328460018811 No Department of Biology, Faculty of Science, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran